Bacterial Biofilm Compressive Raman Imaging
Type d'annonce : Offre d'emploi post-doctorat
De : Laboratoire Jean Perrin , CNRS-Sorbonne I
https://www.labojeanperrin.fr/Documents/Users/nhenry/PhD_Postdoc_Sep2024%20Eng.pdf
Bacterial Biofilm Compressive Raman Imaging
Joint project from Laboratoire Jean Perrin (LJP) and Laboratoire Kastler Brossel (LKB), Biophysics of Micro-Organisms and Optics of Complex Media groups.
PhD, Post-doc, Engineer
Bacterial biofilms are living structures formed by bacteria that attach to surfaces and embed in a self-secreted extracellular matrix (ECM). They have a major impact on human activities and geochemical cycles. We are currently developing a project, aiming to decipher the principles of bacterial biofilm assembly. Jointly supported by LKB and LJP, the objective is to achieve a time-resolved functional mapping of a developing biofilm implementing a combination of conventional and compressive Raman micro-spectroscopies assisted by the use of stable isotope probes (SIP) and complemented by a multiparametric computational analysis. This hybrid strategy should allow a kinetic approach to the biofilm assembly processes from the single cell to the built-up two- and then three-dimensional stages. An AI strategy intended to decipher cell-ECM couplings up to the mature biofilm stage will be implemented. We will start by imaging a simple model biofilm of Escherichia coli, then strains of medical and environmental interest such as Pseudomonas aeruginosa or Bacillus thuringiensis. In the longer term, the completion of the project should make it possible to address the more complex question of the multi-species communities functioning, a key subject of modern microbiology.
This work will require the implementation of microbiology, microfabrication, millifluidics techniques, together with Raman micro-spectroscopy as well as algorithmic processing and modeling approaches in proportions which will depend on the recruited profiles.
Profiles: For this interdisciplinary ANR- funded project, we are recruiting for two PhD, Post-doc and/or engineer fellowships, with physicist and physico-chemist backgrounds trained at the interface with biology. Biologists with strong experience in imaging and quantitative analysis may also be considered. Experience in Raman spectroscopy would be greatly appreciated.
Location: The two host laboratories are located in the 5th arrondissement in the center of Paris and approximately 15 min walk.
Recruitment: Candidates will be recruited in one or the other of the two laboratories according to their profile with a main assignment but they will work in both laboratories. The salary will depend on the level (approx. between €2100 and €3600 gross salary). CVs with 2 recommandation letters are welcome by email at the following contacts. Interviews will begin mid-september 2024 for a flexible hiring date no earlier than November 1, 2024.
Supervision and contact:
nelly.henry@sorbonne-universite.fr h.aguiar@phys.ens.fr
Micro-Organisms Biophysics (MOB) Optical imaging in complex media (here)
Laboratoire Jean Perrin (LJP) Laboratoire Kastler Brossel (LKB) Campus Pierre et Marie Curie Campus ENS-Ulm
4, Place Jussieu, Paris 5e 24 rue Lhomond, Paris 5e
Détails de l'annonce
- Profil : Biophysicist - Physicist - Physico-chimist
- Durée de contrat : 2ans
- Date de prise de fonction : Flexible from Nov 1st, 2024
- Documents à fournir pour candidater : CV, Lettre de motivation, Lettre de recommandation
- Date limite de candidature : 30/04/2025
Personne à contacter
- Nom : Nelly Henry
- Email : nelly.henry@sorbonne-universite.fr